anti noggin goat polyclonal anti serum  (R&D Systems)

Journal: eLife

Article Title: Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta

doi: 10.7554/eLife.52690

Figure Lengend Snippet:

Article Snippet: Antibody , anti-mouse NOGGIN (goat polyclonal) , R and D systems , Cat #: AF719 RRID: AB_2151669 , 1:200 , .

Techniques: Sequencing, Purification, Agarose Gel Electrophoresis, Software

Journal: PLoS ONE

Article Title: Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins

doi: 10.1371/journal.pone.0214758

Figure Lengend Snippet: Anterior lens tissue was double labeled with the G8 mAb (green in A and D) and polyclonal antibodies to filensin (Fil) or CP49 (red in B and E). Nuclei were stained with Hoechst dye (blue). Overlap of green and red appears yellow in merged images (C and F). The subpopulation of G8+ cells bound antibodies to beaded filament proteins (C and F). The inset in F shows G8+/CP49- cells. Lens tissue double labeled with the anti-mouse IgM and anti-rabbit Ig secondary antibodies alone were unstained (inset in C). Bar = 9 μm.

Article Snippet: Anterior lens tissue, cultured RMS cells and tumor tissue sections were double labeled with the G8 IgM mouse mAb [ ] diluted 1:40 and a goat polyclonal antiserum to noggin (AF719; R&D Systems, Minneapolis, MN) diluted 1:100, rabbit polyclonal antisera to filensin [ ] diluted 1:1000, or a mouse mAb to MyoD (MA5-12902; ThermoFisher Scientific/Invitrogen) diluted 1:100 by previously described methods [ , , ].

Techniques: Labeling, Staining

Journal: PLoS ONE

Article Title: Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins

doi: 10.1371/journal.pone.0214758

Figure Lengend Snippet: Cultures of RC13 ARMS (A-C, G-I and M-O) and RD ERMS cells (D-F, J-L and P-R) were double labeled with the G8 mAb (red) and polyclonal antibodies to noggin (Nog), filensin (Fil) or CP49 (green). Nuclei were stained with Hoechst dye (blue). Overlap of red and green appears yellow in merged images (C, F, I, L, O and R). Most RC13 ARMS cells and a subpopulation of RD ERMS cells bound antibodies to G8, noggin, filensin and CP49. Bar = 9 μm.

Article Snippet: Anterior lens tissue, cultured RMS cells and tumor tissue sections were double labeled with the G8 IgM mouse mAb [ ] diluted 1:40 and a goat polyclonal antiserum to noggin (AF719; R&D Systems, Minneapolis, MN) diluted 1:100, rabbit polyclonal antisera to filensin [ ] diluted 1:1000, or a mouse mAb to MyoD (MA5-12902; ThermoFisher Scientific/Invitrogen) diluted 1:100 by previously described methods [ , , ].

Techniques: Labeling, Staining

Journal: PLoS ONE

Article Title: Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins

doi: 10.1371/journal.pone.0214758

Figure Lengend Snippet: Transblots of proteins extracted from RC13 human RMS cells and mouse lens tissue were probed with polyclonal antibodies to filensin (Fil) and CP49. Beaded filament antibodies bound proteins of similar molecular weights in RMS and lens cells.

Article Snippet: Anterior lens tissue, cultured RMS cells and tumor tissue sections were double labeled with the G8 IgM mouse mAb [ ] diluted 1:40 and a goat polyclonal antiserum to noggin (AF719; R&D Systems, Minneapolis, MN) diluted 1:100, rabbit polyclonal antisera to filensin [ ] diluted 1:1000, or a mouse mAb to MyoD (MA5-12902; ThermoFisher Scientific/Invitrogen) diluted 1:100 by previously described methods [ , , ].

Techniques:

Journal: PLoS ONE

Article Title: Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins

doi: 10.1371/journal.pone.0214758

Figure Lengend Snippet: Sections of human ARMS of the nose, ERMS from an intrabdominal tumor, spindle cell RMS (SCRMS) from the testis, pleomorphic RMS (PRMS) of the retroperitoneum and Wilms (kidney) tumors were double labeled with the G8 mAb (red) and polyclonal antibodies to filensin (green). Nuclei were stained with Hoechst dye (blue). Overlap of red and green appears yellow in merged images. RMS and Wilms tumor sections contained subpopulations of cells double labeled with antibodies to G8 and filensin. Bar = 9 μM.

Article Snippet: Anterior lens tissue, cultured RMS cells and tumor tissue sections were double labeled with the G8 IgM mouse mAb [ ] diluted 1:40 and a goat polyclonal antiserum to noggin (AF719; R&D Systems, Minneapolis, MN) diluted 1:100, rabbit polyclonal antisera to filensin [ ] diluted 1:1000, or a mouse mAb to MyoD (MA5-12902; ThermoFisher Scientific/Invitrogen) diluted 1:100 by previously described methods [ , , ].

Techniques: Labeling, Staining, Wilms Tumor Assay